|
|
|
| Registros recuperados: 49 | |
|
|
| Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These... |
| Tipo: Text |
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf |
| |
|
| |
|
|
| Munck, Nanna; Leekitcharoenphon, Pimlapas; Litrup, Eva; Kaas, Rolf; Meinen, Anika; Guillier, Laurent; Tang, Yue; Malorny, Burkhard; Palma, Federica; Borowiak, Maria; Gourmelon, Michele; Simon, Sandra; Banerji, Sangeeta; Petrovska, Liljana; Dallman, Timothy J.; Hald, Tine. |
| Zoonotic Salmonella causes millions of human salmonellosis infections worldwide each year. Information about the source of the bacteria guides risk managers on control and preventive strategies. Source attribution is the effort to quantify the number of sporadic human cases of a specific illness to specific sources and animal reservoirs. Source attribution methods for Salmonella have so far been based on traditional wet-lab typing methods. With the change to whole genome sequencing there is a need to develop new methods for source attribution based on sequencing data. Four European datasets collected in Denmark (DK), Germany (DE), the United Kingdom (UK) and France (FR) are presented in this descriptor. The datasets contain sequenced samples of Salmonella... |
| Tipo: Text |
|
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00612/72437/71383.pdf |
| |
|
|
| Marti, Romain; Ribun, Sebastien; Aubin, Jean-baptiste; Colinon, Celine; Petit, Stephanie; Marjolet, Laurence; Gourmelon, Michele; Schmitt, Laurent; Breil, Pascal; Cottet, Marylise; Cournoyer, Benoit. |
| Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the most prevalent operating taxonomic units (OTU) to be allocated to Flavobacterium and Aquabacterium. Relative numbers of Gaiella, Haliangium, and Thermoleophilum OTU matched the observed differentiation of bacteriomes according to river compartments.... |
| Tipo: Text |
Palavras-chave: Peri-urban river; Benthic and hyporheic sediments; Microbial community; High throughput sequencing (HTS); Fecal contamination. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00378/48886/49316.pdf |
| |
|
| |
|
|
| Mieszkin, Sophie; Caprais, Marie-paule; Le Mennec, Cecile; Le Goff, Manon; Edge, T. A.; Gourmelon, Michele. |
| Aims: The aim of this study was to identify the origin of faecal pollution impacting the Elorn estuary (Brittany, France) by applying microbial source tracking (MST) markers in both oysters and estuarine waters. Methods and Results: The MST markers used were as follows: (i) human-, ruminant- and pig-associated Bacteroidales markers by real-time PCR and (ii) human genogroup II and animal genogroup I of F-specific RNA bacteriophages (FRNAPH) by culture/genotyping and by direct real-time reverse-transcriptase PCR. The higher occurrence of the human genogroup II of F-specific RNA bacteriophages using a culture/genotyping method, and human-associated Bacteroidales marker by real-time PCR, allowed the identification of human faecal contamination as the... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Estuarine and river waters; F-specific RNA bacteriophages; Host-associated Bacteroidales markers; Microbial source tracking; Shellfish. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00153/26426/25250.pdf |
| |
|
|
| Wieliczko, Etienne; Gourmelon, Michele; Dupray, Elisabeth; Derrien, A.; Caprais, Marie-paule; Pommepuy, Monique. |
| D'un point de vue purement écologique, le milieu marin est particulièrement agressif pour les bactéries d'origine entérique. Jusqu'à la fin des années soixante dix, il était généralement admis que ces bactéries pathogènes étaient détruites en quelques heures dans l'eau de mer, ou selon l'espèce en quelques jours. On parlait alors du "pouvoir auto-épurateur" de l'eau de mer. Depuis des travaux plus récents ont démontré la persistance de microorganismes dans les zones littorales. En fait, la survie en mer des bactéries d'origine fécale dans le milieu marin dépend de deux grands facteurs : la qualité de l'eau ou du sédiment permettant aux bactéries de mettre en oeuvre plus ou moins facilement des mécanismes de "résistance", et leur historique avant leur rejet... |
| Tipo: Text |
|
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00105/21630/19210.pdf |
| |
|
|
| Baliere, Charlotte; Rince, A.; Delannoy, S.; Fach, P.; Gourmelon, Michele. |
| Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin (stx) and intimin (eae) were subtyped using PCR tests and DNA sequencing, respectively. The results showed a high level of diversity between strains, with 17 unique virulence gene profiles for STEC and 56 for EPEC. Seven STEC and 15 EPEC strains were found to display a large... |
| Tipo: Text |
|
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00344/45514/45076.pdf |
| |
|
|
| Rince, Alain; Baliere, Charlotte; Hervio-heath, Dominique; Cozien, Joelle; Lozach, Solen; Parnaudeau, Sylvain; Le Guyader, Francoise S.; Le Hello, Simon; Giard, Jean-christophe; Sauvageot, Nicolas; Benachour, Abdellah; Strubbia, Sofia; Gourmelon, Michele. |
| During a 2-year study, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments. Bacteria isolated from these samples were further analyzed. Escherichia coli isolates classified into the phylogenetic groups B2, or D and enterococci from Enterococcus faecalis and E. faecium species were tested for the presence of virulence genes and for antimicrobial susceptibility. Salmonella members were serotyped and the most abundant serovars (Typhimurium and its monophasic variants and Mbandaka) were genetically characterized by high discriminative subtyping methods.... |
| Tipo: Text |
Palavras-chave: Campylobacter; Salmonella; Vibrio; HuNoVs; Fecal bacterial indicators; Shellfish; Water. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57243/59243.pdf |
| |
|
|
| Derrien, M.; Jarde, E.; Gruau, G.; Pourcher, A. M.; Gourmelon, Michele; Jadas-hecart, Alain; Wickmann, A. C. Pierson. |
| Improving the microbiological quality of coastal and river waters relies on the development of reliable markers that are capable of determining sources of fecal pollution. Recently, a principal component analysis (PCA) method based on six stanol compounds (i.e. 5 beta-cholestan-3 beta-ol (coprostanol), 5 beta-cholestan-3 alpha-ol (epicoprostanol), 24-methyl-5 alpha-cholestan-3 beta-ol (campestanol), 24-ethyl-5 alpha-cholestan-3 beta-ol (sitostanol), 24-ethyl-5 beta-cholestan-3 beta-ol (24-ethylcoprostanol) and 24-ethyl-5 beta-cholestan-3 alpha-ol (24-ethylepicoprostanol)) was shown to be suitable for distinguishing between porcine and bovine feces. In this study, we tested if this PCA method, using the above six stanols, could be used as a tool in... |
| Tipo: Text |
Palavras-chave: Stanols; Microbial Source Tracking; Principal component analysis; Microbial markers. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00090/20133/17829.pdf |
| |
|
|
| Vincent Hubert, Francoise; Wacrenier, Candice; Morga, Benjamin; Lozach, Solen; Quenot, Emmanuelle; Mege, Mickael; Lecadet, Cyrielle; Gourmelon, Michele; Hervio-heath, Dominique; Le Guyader, Soizick. |
| The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species... |
| Tipo: Text |
Palavras-chave: Norovirus; Ostreid herpes virus 1 μ Var; Vibrio spp; Microbial source tracking; Sea; Passive sampler; Oyster (Crassostrea gigas). |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79375/81893.pdf |
| |
|
|
| Harwood, Valerie J.; Boehm, Alexandria B.; Sassoubre, Lauren M.; Vijayavel, Kannappan; Stewart, Jill R.; Fong, Theng-theng; Caprais, Marie-paule; Converse, Reagan R.; Diston, David; Ebdon, James; Fuhrman, Jed A.; Gourmelon, Michele; Gentry-shields, Jennifer; Griffith, John F.; Kashian, Donna R.; Noble, Rachel T.; Taylor, Huw; Wicki, Melanie. |
| An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages... |
| Tipo: Text |
Palavras-chave: Virus; Bacteriophage; Water quality; Fecal pollution; Validation. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00171/28197/27786.pdf |
| |
|
| |
|
|
| Mieszkin, Sophie; Yala, Jean Fabrice; Joubrel, Rozenn; Gourmelon, Michele. |
| Aims: The aims of this study were to evaluate the host-specific distribution of Bacteroidales 16S rRNA gene sequences from human- and animal-related effluents and faeces, and to define a ruminant-specific marker. Methods and Results: Bacteroidales 16S rRNA gene clone libraries were constructed from samples of effluent (sewage, bovine manure and pig slurry) and faeces (human, bovine, pig and wild bird), using PCR primers targeting order Bacteroidales. The phylogenetic analysis revealed six main distinct human-, bovine-, pig- and wild bird-specific clusters. From the bovine-specific cluster II, we designed a ruminant-specific marker, Rum-2-Bac, and this showed 97% sensitivity (n = 30) and 100% specificity (n = 40) when tested by TaqMan (R) real-time PCR.... |
| Tipo: Text |
Palavras-chave: 16S rRNA gene; Bacteroidales; Faecal contamination; Microbial Source Tracking; Sewage and manure. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00000/11156/8235.pdf |
| |
|
|
| Caprais, Marie-paule; Le Mennec, Cecile; Pommepuy, Monique; Le Saux, Jean-claude; Gourmelon, Michele. |
| In Europe, shellfish harvesting areas are classified according European Directive EC N°854/2004, using classic faecal indicator levels (Escherichia coli). A better indicator is needed, however, because shellfish may contain other human pathogens. Other indicator organisms have therefore been evaluated and, among these, coliphages meet most of the requirements to serve as indicators and models for enteroviruses. In order to evaluate the capacity of coliphages as indicators, a total of 764 shellfish were sampled in 2004 from 70 shellfish harvesting areas monitored by the Ifremer microbiological surveillance network (REMI, http://www.ifremer.fr/envlit/), and tested for F-specific RNA bacteriophages (FRNAPH). Two species of bivalve mollusc were collected... |
| Tipo: Text |
Palavras-chave: F-specific RNA bacteriophage; Shellfish; Faecal pollution; Discrimination. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17749/15271.pdf |
| |
|
|
| Gourmelon, Michele; Lozach, Solen; Garry, Pascal; Balliere, Clémence; Sauvageot, Nicolas; Le Hello, Simon; Rince, Alain. |
| Shellfish farming is an important economic activity in the Brittany and Normandy regions. However, a part of the production sites corresponds to relatively sensitive areas where the presence of faecal microorganisms is a major concern for shellfish and constitutes a possible health risk. Indeed, shellfish bioaccumulates in their tissues pathogenic contaminants present in water and can cause food-borne diseases such as salmonellosis. During a two-year study, we evaluated the presence of faecal indicators, measured the prevalence of Salmonella spp., isolated and characterized Salmonella spp. from three French shellfish-harvesting areas (shellfish and sediment) and their watersheds (from river water samples). |
| Tipo: Text |
|
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00341/45199/44609.pdf |
| |
|
| |
| Registros recuperados: 49 | |
|
|
|
